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pump1
|
Rotenone
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pump3
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Antimycin
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pump4
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Cyanideco
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ATP synthase
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Oligomycin
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protons
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uncoupling agent
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Glycolysis
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ATP
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2
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|
NADH
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3-5
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Pyruvate metabolism
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NADH
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5
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|
TCA cycle
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ATP
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2
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NADH
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6
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|
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FADH
2 |
2
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NADH-Q Oxidoreducatase
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Enormous enzyme (>900 kDa), 46 polypeptides
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FMN, Fe-S clusters
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Steps of Electron-Transfer:
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1. Binding of NADH and transfer of its electrons to FMN (prosthetic group of complex)
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2. Electrons are transfered from FMNH2 to a series of iron-sulfur clusters (prosthetic group of complex) -> 2Fe-2S + 4Fe-4S clusters
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3. Electrons are shuttled to coenzyme Q (ubiquinone)
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2 Electrons from NADH to Coenzyme Q -> pumping 4 H+ from matrix to intermembrane space
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Electrons Flow from Ubiquinol (QH2) to Cytochrome c Through Q-Cytochrome c Oxidoreductase
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Cytochrome b: heme bL and heme bH
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Cytochrome c1: heme c1
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iron–sulfur protein: 2Fe-2S center
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Function: catalyse transfer of electrons from QH2 -> oxidized cyt c
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pumps 4 H+ out of matrix -> intermembrane space
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Coupling of electron transport from Q -> cyt c and transmembrane proton transport: Q cycle
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Cytochrome c oxidase catalyzes the reduction of molecular oxygen to water
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CuA/CuA, heme a, heme a3, CuB
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heme a3-CuB is responsible for reduction of O2
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Oxidation of cyt c coupled to reduction of O2 H2O
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Electron transfer coupled to proton pump
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pumps 2 H+ from the matrix to intermembrane space
|
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