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Two molecules of carbon dioxide.
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Three molecules of NADH.
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Three hydrogen ions.
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One molecule of FADH₂
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One molecule of GTP.
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The two carbon atoms that enter the cycle as the acetyl group.
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the enzymes of the citric acid cycle are physically associated with one another.
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Substrate channeling; Metabolon
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The cycle operates only under aerobic conditions, although oxygen does not participate directly in the cycle.
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The pyruvate dehydrogenase complex is regulated allosterically and by reversible phosphorylation
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ATP->ADP, H2O->Pi
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The citric acid cycle must be capable of being rapidly replenished if any are drawn off for biosynthesis, to allow the cycle to function
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acetyl CoA can not convert into oxaloacetate or any other citric acid cycle intermediate
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oxaloacetate can be formed by the carboxylation of pyruvate, a crucial role in gluconeogenesis, which is active only in the presence of acetyl CoA
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