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objective
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synthesize ATP (i.e. energy)
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stages
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glycolysis, pyruvate oxidation, krebs/citric acid cycle, electron transport/ oxidative phosphorylation
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equation
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C6H12O6 + 6 O2 + 36 ADP → 6 CO2 + 6 H2O+ 36 ATP
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glycolysis
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in - 1 glucose, 2 NAD, 2 ATP, 4 ADP
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out - 2 pyruvate, 2 NADH, 2 ATP (two consumed in phase one, four produced in phase two)
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pyruvate oxidation
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in - 1 pyruvate, 1 NAD
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out - 1 acetyl-CoA, 1 NADH, 1 CO2
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krebs/citric acid cycle
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in - 1 acetyl-CoA, 3 NAD, 1 FADH, 1 ADP
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out - 1 CoA (acetyl-CoA -> citric acid -> oxaloacetate; oxaloacetate reacts with another acetyl-CoA to form citric acid and repeat cycle), 3 NADH, 1 FADH, 1 ATP, 2 H2O, 1 CO2
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for pyruvate oxidation and krebs cycle, the total number of products should be multiplied by two in order to calculate the number of products per glucose molecules because each process occurs once for each pyruvate formed in glycolysis
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glycolysis
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phosphorylation
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phosphate group is transferred
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isomerization
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molecule is structurally rearranged
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redox
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oxidation/reduction
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lysis/cleavage
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molecule is split into two
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krebs cycle
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phosphorylation
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isomerization
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redox
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decarboxylation
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carboxyl group is removed from molecule; CO2 is produced
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objective
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create a proton gradient by moving hydrogen ions from the mitochondrial matrix to the intermembrane space to drive ATP synthesis
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protein complexes
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four protein complexes
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complex 3 collects electrons from complexes 1 & 2; complex 4 collects electrons from complex 3
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electron shuttles
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NAD(H): NAD is reduced to NADH in previous stages of CR; delivers electrons to complex 1 & 2
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FAD(H2): FAD is reduced to FADH2 in previous stages of CR; delivers electrons to complex 2
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UQ (ubiquinone): shuttles electrons from complexes 1 & 2 to complex 3
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Cyt-c (cytochrome-c): shuttles electrons from complex 3 to 4
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notes
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FADH2 bypasses protein complex 1 because the electrons exist on an energy level that is too low for complex 1 to pick up on
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energy from electrons is used to drive protein complexes; complexes pump hydrogen ions into the intermembrane space
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ATP synthase enzyme pumps one hydrogen ion back into the matrix to synthesize ATP (combines ADP and inorganic phosphate)
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